Scientific Study
Access to over 2,900 scientific references, studies and publications. This section is constantly updated with studies that have been published in scientific journals.
Notice: Undefined variable: post in /home/nimianet/domains/test.nimia.net/public_html/wp-content/mu-plugins/custom-studies.php on line 744
Notice: Trying to get property 'ID' of non-object in /home/nimianet/domains/test.nimia.net/public_html/wp-content/mu-plugins/custom-studies.php on line 744
Identification and in silico bioinformatics analysis of PR10 proteins in cashew nut.
- Journals: Protein Sci
- Pages:
- Year: 2020
Notice: Undefined variable: post in /home/nimianet/domains/test.nimia.net/public_html/wp-content/mu-plugins/custom-articles.php on line 247
Notice: Trying to get property 'ID' of non-object in /home/nimianet/domains/test.nimia.net/public_html/wp-content/mu-plugins/custom-articles.php on line 247
Proteins from cashew nut can elicit mild to severe allergic reactions. Three allergenic proteins have already been identified, and it is expected that additional allergens are present in cashew nut. PATHOGENESIS-RELATED PROTEIN 10 (PR10) allergens from pollen have been found to elicit similar allergic reactions as those from nuts and seeds. Therefore, we investigated the presence of PR10 genes in cashew nut. Using RNA-seq analysis, we were able to identify several PR10-like transcripts in cashew nut and clone six putative PR10 genes. In addition, PR10 protein expression in raw cashew nuts was confirmed by immunoblotting and LC-MS/MS analyses. An in silico allergenicity assessment suggested that all identified cashew PR10 proteins are potentially allergenic and may represent three different isoallergens.