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A high selective immunochromatographic assay for rapid detection of aflatoxin B
Authors: Zhang D, Peiwu L, Yang Y, Zhang Q, Zhang W, Xiao Z, Ding X
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- Journals: Talanta
- Pages:
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To solve the problem of low selectivity of current immunochromatographic assay (ICA) for aflatoxin B1 (AFB1) alone detection; a novel selective ICA was developed here. With very high selectivity; a new AFB1 monoclonal antibody (MAb) 3G1 was preparaed by immunizing Balb/c mice with aflatoxin B2a-BSA (AFB2a-BSA) rather than AFB1-BSA used in other reports and 3G1 possessed the highest selectivity than those used in published ICAs. The ICA with visual detection limit (VDL) of 1 ng mL-1 showed no cross-reactivity with other aflatoxins. Comparing with previous reports; the ICA here provided the most powerful guarantee for avoiding false positive results leaded by coexistence of other aflatoxins in samples. For validation; naturally contaminated samples including peanut; puer-tea; vegetable oil and feedstuff were respectively assayed by ICA and a standard high performance liquid chromatography (HPLC); and good agreement of results was obtained between two methods. Therefore; the developed ICA could well meet the selective detection of AFB1 in agro-products.