Scientific Study
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Evaluation of Extraction Methods for the Identification of Proteins from Date Palm (Phoenix dactylifera L.) Seed and Flesh.
Authors: Lee, H. X., Ahmad, F., Saad, B., & Ismail, M. N.
- Journals: Prep Biochem Biotechnol.
- Pages:
- Year: 2017
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Date fruits are well known to be very nutritious. Nevertheless, the protein content of the fruit, particularly the seed and flesh is still understudied, largely due to their difficult physical characteristics. This study was conducted to compare three different protein extraction methods which were the TCA-acetone (TCA-A), phenol (Phe), and TCA-acetone-phenol (TCA-A-Phe), and to perform proteomic analysis on date palm seed and flesh. Phe extraction method showed the highest protein yields for both seed (8.26 mg/ g) and flesh (1.57 mg/ g). Thru SDS- PAGE, Phe and TCA-A-Phe extraction methods were shown to be efficient in removing interfering compounds and gave well-resolved bands over a wide range of molecular weights. Following LC- MS/MS analysis, about 50- 64% of extracted proteins were identified with known functions including those involved in glycolysis, Krebs cycle, defense, and storage. Phe protein extraction method was proven to be the optimal method for date flesh and seed.