Scientific Study
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Products: Brazil Nuts
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Purification; crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2. Acta Crystallogr Sect F Struct Biol Cryst Commun
Authors: Guo F, Jin T, Howard A, Zhang YZ.
- Journals: null
- Pages: 976-9.
- Volume: 63(Pt 11)
- Year: 2007
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Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study; Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.